In the evaluation of glomerular filtration rate of the children or the small size animals such as rabbits and rats, there are many technically difficult problems in urine collction..
So, clearance was estimated by the calculation of the distributed volume and the disappearance rate in plasma of the indicator.
Constant infusion method provides an effective technique for the evaluation of GFR and. renal plasma flow rate without urine collection.
Comparative experimental analyses between conventional method and constant infusion method in using inulin and hippuran-1131 were made in this experiment operated for the 21 normal male rabbits, weight about 2kg.;of which 10 for inulin clearance (Cin). 11 for hippuran-1111 clearance (CPAH-1131).
The results as follows
1. The mean value of Cin obtained from conventional method was 1.690.59ml/mina The mean value of Cin from theoretical method was 1.450.61ml/min. in the case of 10 rabbits. Correlation coefficient was r=0.948, so regression line was defined by Y=0.916X +0.37. The result shows a correlation was highly significant.
2. The mean value of distributed volume of inulin was 548.10136.60m1, 26.495.42%´-of body weight. But the decay constant was 0.00280.001 by calculated from plasma inulin levels.
3. The mean value of CPAH-1131 obtained from conventional method was 17.55-5.52m1/ min. The mean value of CPAH-1131 from theoretical method was 17.805.79m1/min. in the case of 11 rabbits. Correlation efficient was r=0.948, so regression line was defined by Y=0.903X+1.48. The result shows a correlation was highly significant.
4. The mean value of distributed volume of hippuran-1131 was 565.92119.40m1, but the decay constant was 0.03440.0176 by calculated from plasma hippuran-I131 levels.
|